Lysis:Article Title: Sustaining the T-cell activity in xenografted psoriasis skin
Article Snippet: .. Snap frozen graft biopsies were lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) with Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), Halt TM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. After mechanical lysing, tissue homogenates were centrifuged (15000 g, 4°C, 15 min) and the protein concentration was determined with a BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA).
Article Title: Sustaining the T-cell activity in xenografted psoriasis skin.
Article Snippet: .. Snap frozen graft biopsies were lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) with Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), HaltTM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. After mechanical lysing, tissue homogenates were centrifuged (15000 g, 4 ̊C, 15 min) and the protein concentration was determined with a BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA).
Article Title: Imiquimod induces skin inflammation in humanized BRGSF mice with limited human immune cell activity
Article Snippet: .. Skin biopsies were snap frozen in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) and lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), Halt TM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. The protein concentration was measured by the use of BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA) and adjusted to 2 μg/μl.
Article Title: Imiquimod induces skin inflammation in humanized BRGSF mice with limited human immune cell activity.
Article Snippet: .. Skin biopsies were snap frozen in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) and lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), HaltTM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. The protein concentration was measured by the use of BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA) and adjusted to 2 μg/μl.
Protease Inhibitor:Article Title: Sustaining the T-cell activity in xenografted psoriasis skin
Article Snippet: .. Snap frozen graft biopsies were lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) with Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), Halt TM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. After mechanical lysing, tissue homogenates were centrifuged (15000 g, 4°C, 15 min) and the protein concentration was determined with a BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA).
Article Title: Sustaining the T-cell activity in xenografted psoriasis skin.
Article Snippet: .. Snap frozen graft biopsies were lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) with Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), HaltTM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. After mechanical lysing, tissue homogenates were centrifuged (15000 g, 4 ̊C, 15 min) and the protein concentration was determined with a BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA).
Article Title: Imiquimod induces skin inflammation in humanized BRGSF mice with limited human immune cell activity
Article Snippet: .. Skin biopsies were snap frozen in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) and lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), Halt TM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. The protein concentration was measured by the use of BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA) and adjusted to 2 μg/μl.
Article Title: Imiquimod induces skin inflammation in humanized BRGSF mice with limited human immune cell activity.
Article Snippet: .. Skin biopsies were snap frozen in Precellys Soft tissue homogenizing CK14 tubes (Bertin instruments, Montigny-le-Bretonneux, France) and lysed with a Precellys 24 tissue homogenizer (Bertin instruments, Montigny-le-Bretonneux, France) in Cell signaling lysis buffer (Cell Signaling Technology, Leiden, The Netherlands) added Complete Mini Protease Inhibitor Cocktail Tablet (Roche diagnostics, Mannheim, Germany), HaltTM phosphate inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) and Sodium Orthovanadate (New England Biolabs, Ipswich, MA, USA). .. The protein concentration was measured by the use of BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA) and adjusted to 2 μg/μl.
other:
Article Title: APOE Genotype Influences on The Brain Metabolome of Aging Mice – Role for Mitochondrial Energetics in Mechanisms of Resilience in APOE2 Genotype
Article Snippet: Briefly, weighed brain tissue samples were provided frozen in Precellys soft tissue homogenizing CK14 tubes (Bertin Technologies, Montigny-le-Bretonneux, France).
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